Abstract:

Wet ultra-fine pulverization extraction, microwave-assisted extraction and ultrasound-assisted extraction were used to explore the optimal extraction conditions of water-soluble polysaccharides from Flammulina velutipes. Response surface methodology (RSM) was employed to optimize the conditions of wet ultra-fine pulverization extraction for water-soluble polysaccharides. Results indicated that the optimal extraction processing parameters were the gap of colloid mill at 42μm, extraction temperature of 87 ℃ and extraction time of 170 min. Under the optimal extraction conditions, the extraction rate of polysaccharides was 3.63%. The concentration of water-soluble polysaccharides based on predictive model was 417.6μg/mL, and the actually determined concentration of water-soluble polysaccharides was (418 ± 3.21 )μg/mL.

Key words: Flammulina velutipes, water-soluble polysaccharides, wet ultra-fine pulverization extraction, response surface methodology (RSM)